The present manuscript describes simple, sensitive, rapid, accurate, precise and economical dual wavelength UV Spectrophotometric method for the simultaneous determination of Lafutidine and Rabeprazole Sodium in combined dosage form. The principle for dual wavelength method is “The absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The wavelengths selected for determination of Lafutidine were 281 nm and 287.8 nm, whereas, the wavelengths selected for determination of Rabeprazole Sodium were 269.2 nm and 276.4 nm. Methanol was taken as a solvent. Regression analysis of Beer’s plots showed good correlation in concentration range of 10-45 μg/ml for Lafutidine and 6-22 μg/ml for Rabeprazole Sodium. The mean recovery was found to be 100.35% and 99.33% for Lafutidine and Rabeprazole Sodium, respectively. The precision (intra‐day, inter‐day and repeatability) of method was found to be within the limits as per ICH Guideline Q2(R1). The Proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial Pharmaceutical dosage form.
Loading....